Michael Graves is an Associate Professor, Associate Chair, Department of Biology at UMass Lowell.

Michael V. Graves

Associate Professor

College
College of Sciences
Department
Biological Science
Phone
978-934-2887
Email
Michael_Graves@uml.edu

Expertise

Molecular Biology and Virology

Research Interests

Molecular Biology and Virology

Research in my lab focuses on the chlorella viruses that infect chlorella-like algae. They are distributed worldwide and many genetically distinct isolates populate a single water sample. Their genome is unusually large. One isolate, PBCV-1, for example, (genome size = 330744 bp dsDNA) codes for ~375 proteins, nearly half of which resemble proteins of known function. The genomes of other chlorella virus isolates are probably co-linear with PBCV-1 and appear to share many, but not all, genes. This suggests that the entire "gene pool" of this genus exceeds the 375 PBCV-1-encoded genes. Many of these genes encode proteins (e.g., enzymes involved in protein glycosylation) rarely associated with viruses.

Education

  • Ph D: Molecular Genetics, Virology, (1992), Oregon State University - Oregon
    Dissertation/Thesis Title: Analysis of two highly expressed genes from Chlorella virus PBCV-1: Protein characterization and the DNA sequences of the major capsid protein gene and the early/late 33-kDa protein gene.
  • BS: Biology, (1986), University of Nebraska, Lincoln. - Lincoln, Nebraska

Selected Awards and Honors

  • Teaching Appreciation Award (2008) - Student Government Association, University of Massachusetts, Lowell
  • Teaching Excellence Award (2005) - Department of Biological Sciences, University of Massachusetts, Lowell.
  • Phi Beta Kappa (1986) - University of Nebraska, Lincoln, Alpha Chapter

Selected Publications

  • Schneider, W.L., Damsteegt, V.D., Stone, A.L., Kuhlmann, M., Bunyard, B.A., Sherman, D.J., Graves, M.V., Smythers, G., Smith, O.P., Hatziloukas, E. (2011). Molecular Analysis of Soybean Dwarf Virus Isolates in the Eastern United States Confirms the Presence of Both D and Y Strains and Provides Evidence of Mixed Infections and Recombination. Virology, 412(1) 46-54.
  • Van Etten, J.L., Gurnon, J.R., Yanai-Balser, G.M., Dunigan, D.D., Graves, M.V. (2010). Chlorella Viruses Encode Most, If Not All, of the Machinery to Glycosylate Their Glycoproteins Independent of the Endoplasmic Reticulum and Golgi. Biochimica et Biophysica Acta - General Subjects, 1800(2) 152-159.
  • Nguyen, P.s., Falcone, D.L., Graves, M.V. (2010). The A312L 5'-UTR of Chlorella Virus PBVC-1 is a Translational Enhancer in Arabidopsis Thaliana. Virus research, 140(1-2) 138-146.
  • Graves, M.V., Van Etten, J.L. (2008). Algal Viruses (Phycodnaviridae) (1:). Virology
  • Fitzgerald, L.A., Boucher, P.T., Yanai-Balser, G.M., Suhre, K., Graves, M.V., Van Etten, J.L. (2008). Putative Gene Promoter Sequences in the Chlorella Viruses. Virology, 380(2) 388-393.
  • Tchantchou, F., Graves, M.V., Falcone, D., Shea, T.B. (2008). S-Adenosylmethionine Mediates Glutathione Efficacy by Increasing Glutathione S-Transferase Activity: Implications for S-Adenosyl Methionine as a Neuroprotective Dietary Supplement. Journal of Alzheimers Disease, 14(3).
  • Shaughnessy, P., Graves, M.V., Livingston, G. (2008). Towards Predicting Protein-Protein Interactions in Novel Organisms. International journal of computational biology and drug design, 1(3) 235-253.
  • Graves, M.V., Fitzgerald, L.A., Li, X., Hartigan, J., Pfitzner, A.J., Hoffart, E., Van Etten, J.L. (2007). Corrigendum to "Sequence and Annotation of the 288-kb ATCV-1 Virus That Infects an Endosymbiotic Chlorella Strain of the Heliozoon Acanthocystis Turfacea" . Virology, 366(1) 226.
  • Graves, M.V., Fitzgerald, L.A., Li, X., Hartigan, J., Pfitzner, A.J., Hoffart, E., Van Etten, J.L. (2007). Sequence and Annotation of the 288-kb ATCV-1 Virus That Infects an Endosymbiotic Chlorella Strain of the Heliozoon Acanthocystis Turfacea. Virology, 362(2) 350-361.
  • Fitzgerald, L.A., Graves, M.V., Li, X., Feldblyum, T., Hartigan, J., Van Etten, J.L. (2007). Sequence and Annotation of the 314-Kb MT325 and the 321-kb FR483 Viruses That Infect Chlorella Pbi. Virology, 358 459-471.
  • Fitzgerald, L.A., Graves, M.V., Li, X., Feldblyum, T., Nierman, W.C., Van Etten, J.L. (2007). Sequence and annotation of the 369-kb NY-2A and the 345-kb AR158 viruses that infect Chlorella NC64A. Virology, 358 472-484.
  • Tchantchou, F., Graves, M.V., Shea, T.B. (2006). Expression and Activity of Methionine Cycle Genes Are Altered Following Folate and Vitamin E Deficiency Under Oxidative Challenge: Modulation by Apolipoprotein E-Deficiency. Nutritional Neuroscience, 9(1-2) 17-24.
  • Tchantchou, F., Graves, M.V., Ortiz, D., Chan, A., Rogers, E., Shea, T.B. (2006). S-Adensosyl Methionine: A Connection Between Nutritional and Genetic Risk Factors for Neurodegeneration in Alzheimer's Disease. J. Nut. Health and Aging, 10 541-544.
  • Tchantchou, F., Graves, M.V., Rogers, E., Ortiz, D., Shea, T.B. (2005). N-Acetyl Cysteine Alleviates Oxidative Damage to Central Nervous System of Apoe-Deficient Mice Following Folate and Vitamin E-Deficiency. Journal of Alzheimer's Disease, 7(2) 135-138.
  • Lee, D.Y., Graves, M.V., Van Etten, J.L., Choi, . (2005). T-J Functional Implication of the tRNA Genes Encoded in the Chlorella Virus Pbcv-1 Genome. Plant Pathol J., 21 334-342.
  • Tchantchou, F., Graves, M.V., Ortiz, D., Rogers, E., Shea, T.B. (2004). Dietary Supplementation with 3-Deaza Adenosine, N-Acetyl Cysteine, and S-Adenosyl Methionine Provide Neuroprotection Against Multiple Consequences of Vitamin Deficiency and Oxidative Challenge. Relevance to Age-Related Neurodegeneration. NeuroMolecular Medicine, 6(2-3) 93-104.
  • Kang, M., Graves, M.V., Mehmel, M., Moran, A., Gazzarrini, A., Thiel, G., Gurnon, J.R., Van Etten, J.L. (2004). Genetic Diversity in Chlorella Viruses Flanking KCV, a Gene that Encodes a Potassium Ion Channel Protein. Virology, 356 150-159.
  • Tchantchou, F., Graves, M.V., Ashline, D., Morin, A., Pimenta, A., Ortiz, D., Rogers, E., Shea, T.B. (2004). Increased Transcription and Activity of Glutathione Synthase in Response to Deficiencies in Folate, Vitamin E, and Apolipoprotein E. Journal of neuroscience research, 75(4) 508-15.
  • Tchantchou, F., Graves, M.V., Ortiz, D., Rogers, E., Shea, T.B. (2004). Interaction of Folate Deficiency and Apolipoprotein E Gene Dosage on Cognitive Impairment and Oxidative Damage to Brain Tissue: Compensatory Regulation of Methionine Cycle Genes. Neurobiology of aging, 25.
  • Van Etten, J.L., Graves, M.V., Mueller, D.G., Bolland, W., Delaroque, N. (2002). Phycodnaviridae- Large DNA Algal Viruses. Archives in Virology, 147 1479-1516.
  • Nandhagopal, N., Simpson, A.A., Gurnon, J.R., Yan, X., Baker, T.S., Graves, M.V., Van Etten, J.L., Rossmann, M.G. (2002). The Structure and Evolution of the Major Capsid Protein of a Large, Lipid-Containing DNA Virus. Proceedings of the National Academy of Sciences of the United States of America, 99(23) 14758-14763.
  • Graves, M.V., Bernadt, C.T., Cerny, R., Van Etten, J.L. (2001). Molecular and Genetic Evidence for a Virus-Encoded Glycosyltransferase involved in Protein Glycosylation. Virology, 285 332-345.
  • Sun, L., Gurnon, J.R., Kutish, G., Graves, M.V., Van Etten, J.L. (2000). Characterization of a β-1, 3-Glucanase Encoded by Chlorella Virus PBCV-1. Virology, 276 27-36.
  • Kaiser, A., Vollmert, M., Tholl, D., Graves, M.V., Gurnon, J.R., Xing, W., Lisec, A.D., Nickerson, K.W., Van Etten, J.L. (1999). Chlorella Virus PBVC-1 Encodes a Functional Homospermidine Synthase. Virology, 263 254-262.
  • Graves, M.V., Burbank, D.E., Roth, R., Heuser, J., DeAngelis, P.L., Van Etten, J.L. (1999). Hyaluronan Synthesis in Virus BVC-1 Infected Chlorella-Like Green Algae (257:1 pp. 15-23). Virology
  • Landstein, D., Graves, M.V., Burbank, D.E., DeAngelis, P.L., Van Etten, J.L. (1998). Chlorella Virus PBCV-1 Encodes Functional Glutamine: Fructose-6-Phosphate Amidotransferase and UPD-Glucose Dehydrogenase Enzymes. Virology, 250 388-396.
  • Graves, M.V., Pogany, J., Romero, J. (1997). Defective Interfering RNAS and Defective Viruses Associated with Multipartite RNA Viruses of Plants (7: pp. 399-408). Seminars in Virology
  • DeAngelis, P.L., Jing, W., Graves, M.V., Burbank, D.E., Van Etten, J.L. (1997). Hyaluronan Synthase of Chlorella Virus PBCV-1. Science, 278 1800-1803.
  • Graves, M.V., Roossinck, M.J. (1995). Characterization of Defective RNAs Derived from RNA 3 of the Fny Strain of Cucumber Mosaic Cucumovirus. Journal of Virology, 69 4746-4751.
  • Graves, M.V., Meints, R.H. (1992). Characterization of the Gene Encoding the Most Abundant in Vitro Translation Product from Virus-Infected Chlorella-Like Algae. Gene, 113 149-155.
  • Graves, M.V., Meints, R.H. (1992). Characterization of the Major Capsid Protein and Cloning of Its Gene from Algal Virus PBCV-1. Virology, 188 198-207.
  • McCluskey, K., Graves, M.V., Mills, D., Meints, R.H. (1992). Replication of Chlorella Virus PBCV1 and Host Karyotype Determination Studied with Pulse-Field Gel Electrophoresis . Journal of Psychology, 28 846-850.
  • Schuster, A.M., Graves, M.V., Korth, K., Zieglebein, M., Brumbaugh, J., Grone, D., Meints, R.H. (1990). Transcription and Sequence Studies of a 4.3kbp Fragment from a Ds-DNA Eukaryotic Algal Virus. Virology, 176 515-523.

Selected Presentations

  • Are Viruses Alive? - General Virology lecture to sophomore biology students, 2012 - Kingston, NH
  • - Fundamentals in Biotechnology Workshop, 2011
  • Towards Predicting Protein-Protein Interactions in Novel Organisms - IEEE 7th International Conference on BioInformatics and BioEngineering, 2007 - Harvard Medical School, Boston, MA
  • Algal Virus Genomics - Inter-campus Marine Science Symposium, 2007 - Lowell, Massachusetts
  • Algal Virus Genomics - Microbial Genome Sequencing Annual Meeting, Plant & Animal Genome Conference, 2006 - San Diego, CA
  • Characterization of a Chlorella Virus Encoded Putative beta-lactamase Protein. - New England Biology Conference, 2006 - Boston, MA
  • Does PBCV-1 mRNA Contain a Translational Enhancer? - New England Biology Conference, 2006 - Boston, MA
  • Sequence Analysis of Two Large Chlorella Viruses - 4th Algal Virus Workshop, 2005 - Amsterdam, The Netherlands
  • Adaptations to host and transmission method in Plum pox potyvirus - American Society for Virology Annual Meeting, 2005 - Penn State University, University Park, PA
  • Introducing Freshman Biology Majors to Information Literacy - Conversations on Teaching and Learning: "Teaching with Technology", 2005
  • Genomic Comparisons of Chlorella Viruses to the 1.2 Megabase Mimivirus - Society for Virology Annual Meeting, 2005 - Penn State University, University Park, PA
  • Expression of recombinant A189/92 protein of PBCV-1 and the importance of tRNA genes encoded in the viral genome - 23rd American Society for Virology Meeting, 2004 - Montreal, Quebec, Canada
  • Folate deficiency alters expression of methionine cycle genes: Impact of apolipoprotein E gene dosage, 2004 - Boston, MA
  • Algal Virus Genomics - Microbial Genome Sequencing Program Awardees Workshop, 2004 - Arlington, VA
  • Functional Genmoics of a Virus: The Unusual Lifestyle of the Chloroviruses, 2003
  • Functional Genmoics of a Virus: The Unusual Lifestyle of the Chloroviruses - Biology Department Seminar, 2002 - Boston
  • Functional Genmoics of a Virus: The Unusual Lifestyle of the Chloroviruses - Environmental Biology Seminar, 2002
  • Functional Genmoics of a Virus: The Unusual Lifestyle of the Chloroviruses, 2002 - Indianapolis, IN
  • Structure of a large (T = 169) icosahedral dsDNA virus - 20th American Society for Virology Meeting, 2001 - Madison, WI
  • A PBCV-1-encoded glycosyltransferase involved in glycosylation of its major capsid protein - 19th American Society for Virology Meeting, 2000 - Ft. Collings, CO
  • Alteration of the Host Cell-Wall Surface During Chlorella Virus PBCV-1 Infection - 2nd Algal Virus Workshop, 2000 - Galway, Ireland
  • - Comparative Pathobiology / Plant Pathology Seminar, 2000
  • Genetic Analysis of Polyglycan Synthesis by Chlorella Virus PBCV-1: Chlorella Viruses, so sweet yet so misunderstood, 2000 - Bloomington, IN
  • Genetic Analysis of Polyglycan Synthesis by Chlorella Virus PBCV-1: Hyaluronan Synthesis and Protein Glycosylation, 2000 - Athens, GA
  • Genetic Analysis of Polyglycan Synthesis by Chlorella Virus PBCV-1: Hyaluronan Synthesis and Protein Glycosylation, 2000 - Columbia, MO
  • Viral encoded homospermidine synthesis in a Chlorella-like green algae - 18th American Society for Virology Meeting, 1999 - Amherst, MA
  • Structure and glycosylation of the major capsid protein of Chlorella-virus PBCV-1 - Plant Sciences Retreat, 1999
  • Hyaluronan synthesis in a virus infected algae - 17th American Society for Virology Meeting, 1998 - Vancouver, B.C., Canada
  • - Plant Biology Division 10th Anniversary Plant Biology Symposium, 1998 - Ardmore, OK
  • Chlorella virus PBCV-1 contains genes that encode functional proteins involved in sugar manipulation - 16th American Society for Virology Meeting, 1997 - Bozeman, MT
  • De novo generation of defective RNAs from cucumber mosaic virus - 15th American Society for Virology Meeting, 1996 - London, Ontario, Canada
  • , 1996 - DeKalb, IL
  • Host specific maintenance of a cucumovirus defective RNA - 14th American Society for Virology Meeting, 1995 - Austin, TX
  • Isolation and characterization of a new DI RNA derived from the Fny strain of cucumber mosaic cucumovirus - 13th American Society for Virology Meeting, 1994 - Madison, WI
  • Biochemical analysis of the replication of cucumber mosaic virus strains Fny and Sny in muskmelon - 12th American Society for Virology Meeting, 1993 - Davis, CA

Selected Contracts, Fellowships, Grants and Sponsored Research

  • Algae Bioreactor Evaluation II (), Contract - Algae Grow Lab, Wakefield, MA
    Graves, M. (Principal)
  • Algal Bioreactor Evaluation I (), Contract - Algae Grow Lab, Wakefield, MA
    Graves, M. (Principal)
  • Chlorell spp. Genome Sequencing (), Grant - Joint Genome Institute, Department of Energy
    Graves, M.
  • Determining Protein-Protein Interactions in Virus Infected Chlorella-Like Green Algae (), Grant - UMass Lowell Seed Funding Program to Advance Research and Scholarship
    Graves, M.
  • Microeukaryote diversity in the Gulf of Maine: Under-explored microbial diversity in a well-studied coastal marine ecosystem (), Grant - International Census of Marine Sciences
    Graves, M. (Co-Principal)
  • Algae Photobioreactor Evaluation (2012), -
    Graves, M.V. (Principal)
  • Algae Photobioreactor Evaluation (2011), -
    Graves, M.V. (Principal)
  • Algal Virus Genomics (2003), Grant - NSF: Microbial Genome Sequencing Program
    Graves, M. (Principal)
  • Defective RNA Formation and Recombination in Bromoviruses (1995), - S. R. Noble Foundation Postdoctoral Excellence Award, Northern Illinois University
    Graves, M.