Strain and Cell Line Increased Expression Development:
E. coli, P. pastoris, S. cerevisae, and CHO.
For project data: Intracellular soluble; Periplasmic secreted Case studies will be available soon on these subjects.
Fermentation and Cell Culture Development:
- Media formulation and optimization
- Experimental design
- Optimization of feeding strategies and induction
Harvest and Purification:
- TFF membrane processes (UF/MF)
- Depth filtration
- Expanded bed absorption
- Process chromatography (ion exchange, HIC, affinity, size exclusion, HA, etc.)
Process Optimization:
- Optimize operating parameters
- Identify “key” process parameters for further characterization
Process Characterization:
- Create scale-down models
- Determine acceptable ranges for “key” process parameters
- Create list of critical process parameters (CPP) for validation