02/17/2025
By Kwok Fan Chow
Degree: Doctoral
Location: Olney Hall, Room 520
Date: Monday, March 3, 2025
Time: 10 a.m.
Committee Chair:
Professor Suri Iyer, Department of Chemistry, University of Massachusetts Lowell
Committee Members:
Professor Matthew Gage, Department of Chemistry, University of Massachusetts Lowell
Professor. Micheal Ross, Department of Chemistry, University of Massachusetts Lowell
Professor Marina Ruths, Department of Chemistry, University of Massachusetts Lowell
Abstract:
Biomarkers are essential for early disease detection, offering the highest likelihood of successful treatment. However, current detection methods often lack the sensitivity and affordability needed to fully realize their potential, particularly in resource-limited settings. Human immunodeficiency virus (HIV), affecting over 40 million individuals globally, requires early and accurate diagnosis for effective management. The HIV-1 p24 antigen is a key biomarker for early-stage detection, yet existing diagnostic methods often fail to achieve the necessary sensitivity. This proposal aims to develop an ultrasensitive assay for detecting the HIV-1 p24 antigen, utilizing bioorthogonal chemistry and layer-by-layer signal amplification with Rhodamine B isothiocyanate (RITC)-encapsulated nanoparticles. This innovative approach will enhance signal strength, reduce the limit of detection (LOD), and expand the linear detection range, ensuring reliable detection of low-abundance biomarkers.
Furthermore, we also aim to detect inflammatory biomarkers, including Tumor Necrosis Factor Alpha (TNF-α) and Interleukin 10 (IL-10). By employing a nanoparticle-based assay with bioorthogonal chemistry, we aim to achieve femtomolar sensitivity and validate the results against standard ELISA techniques. The proposed assay's exceptional sensitivity and broad detection range will establish it as a versatile tool for both HIV diagnostics and the monitoring of inflammatory diseases.
All interested students and faculty members are invited to attend.