Id: 001233
Credits Min: 4
Credits Max: 4
Description
A series of lecture and laboratory exercises that will focus on the in vitro culture and analysis of multiple cell type commonly used in biomedical research laboratories. The lecture component will review methodologies used to establish immortalized cell lines, medium component for specific cell types, and techniques for genetically manipulating and analyzing cell lines. The laboratory exercises will emphasize the mastery of sterile techniques used to grow both established cell line and primary cultures, and molecular tools used for introducing recombinant genes and for analyzing cell growth and differentiation.
Prerequisites
BIOL.2200 Principles of Cellular Biology, and BIOL.4210L or Biol.5210L Biochemistry Techniques.
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